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ProductsBack/Vesicular Acetylcholine Transporter antibody

Vesicular Acetylcholine Transporter antibody

GTX31136
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
TargetSLC18A3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Vesicular Acetylcholine Transporter antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 10 - 50 microg/ml. ICC/IF: 1:10 - 1:500. IHC: 10 - 50 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6572
  • Target name
    SLC18A3
  • Target description
    solute carrier family 18 member A3
  • Target synonyms
    CMS21; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18, member 3; VACHT; vesicular acetylcholine transporter
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16572
  • Protein Name
    Vesicular acetylcholine transporter
  • Scientific Description
    This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC18A3 using anti-SLC18A3 antibody (A04891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysates, lane 2: HEPA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC18A3 antigen affinity purified polyclonal antibody (Catalog # A04891) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC18A3 at approximately 55KD. The expected band size for SLC18A3 is at 57KD.
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