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Formalin-fixed and paraffin embedded rat brain labeled with Anti-VGLUT2 Polyclonal Antibody, Unconjugated (bs-9686R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-VGLUT2 Polyclonal Antibody, Unconjugated (bs-9686R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-VGLUT2 Polyclonal Antibody, Unconjugated (bs-9686R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

VGLUT2 Polyclonal Antibody

BS-9686R
Bioss
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC17A6
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Overview

  • Supplier
    Bioss
  • Product Name
    VGLUT2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID57084
  • Target name
    SLC17A6
  • Target description
    solute carrier family 17 member 6
  • Target synonyms
    DNPI, VGLUT2, vesicular glutamate transporter 2, differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 (vesicular glutamate transporter), member 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P2U8
  • Protein Name
    Vesicular glutamate transporter 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VGLUT2/SLC17A6 using anti-VGLUT2/SLC17A6 antibody (A04376-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VGLUT2/SLC17A6 antigen affinity purified polyclonal antibody (Catalog # A04376-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VGLUT2/SLC17A6 at approximately 64 kDa. The expected band size for VGLUT2/SLC17A6 is at 64 kDa.
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