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Virus Induced Signaling Adapter (VISA) Polyclonal Antibody

CAU35808
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetMavs
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Overview

  • Supplier
    Biomatik
  • Product Name
    Virus Induced Signaling Adapter (VISA) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID228607
  • Target name
    Mavs
  • Target description
    mitochondrial antiviral signaling protein
  • Target synonyms
    D430028G21Rik, IPS-1, Visa, cardif, mitochondrial antiviral-signaling protein, CARD adapter inducing interferon beta, IFN-beta promoter stimulator-1, interferon beta promoter stimulator protein 1, mitochondrial anti-viral signaling protein, virus-induced signaling adapter
  • Host
    Rabbit
  • Protein IDQ8VCF0
  • Protein Name
    Mitochondrial antiviral-signaling protein
  • Scientific Description
    The Virus Induced Signaling Adapter (VISA) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mavs using anti-Mavs antibody (A00169-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mavs antigen affinity purified polyclonal antibody (Catalog # A00169-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mavs at approximately 57 kDa. The expected band size for Mavs is at 57 kDa.
Product group Antibodies
Boster Bio
Anti-Mavs Antibody Picoband(r)A00169-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetMavs
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