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HEK 239T cells probed with VLDL Receptor Polyclonal Antibody, Unconjugated (bs-4228R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
HEK 239T cells probed with VLDL Receptor Polyclonal Antibody, Unconjugated (bs-4228R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
HEK 239T cells probed with VLDL Receptor Polyclonal Antibody, Unconjugated (bs-4228R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

VLDL Receptor Polyclonal Antibody

BS-4228R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetVLDLR
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Overview

  • Supplier
    Bioss
  • Product Name
    VLDL Receptor Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7436
  • Target name
    VLDLR
  • Target description
    very low density lipoprotein receptor
  • Target synonyms
    CAMRQ1, CARMQ1, CHRMQ1, VLDL-R, VLDLRCH, very low-density lipoprotein receptor, VLDL receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98155
  • Protein Name
    Very low-density lipoprotein receptor
  • Reactivity
    Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA025865LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of VLDL Receptor/VLDLR using anti-VLDL Receptor/VLDLR antibody (A02195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VLDL Receptor/VLDLR antigen affinity purified polyclonal antibody (Catalog # A02195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VLDL Receptor/VLDLR at approximately 100 kDa. The expected band size for VLDL Receptor/VLDLR is at 100 kDa.
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