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Western Blot Positive WB detected in: PC-3 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: vnn1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: vnn1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: vnn1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa

VNN1 Antibody

CSB-PA025883LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVNN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    VNN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8876
  • Target name
    VNN1
  • Target description
    vanin 1
  • Target synonyms
    HDLCQ8, Tiff66, pantetheinase, pantetheine hydrolase, vannin 1, vascular non-inflammatory molecule 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95497
  • Protein Name
    Pantetheinase
  • Scientific Description
    Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VNN1 using anti-VNN1 antibody (PB10106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SKOV3 whole cell lysates, Lane 2: HELA whole cell lysates lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VNN1 antigen affinity purified polyclonal antibody (Catalog # PB10106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VNN1 at approximately 57 kDa. The expected band size for VNN1 is at 57 kDa.
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