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VPS41 Antibody

LS-C661571
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetVPS41
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    VPS41 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27072
  • Target name
    VPS41
  • Target description
    VPS41 subunit of HOPS complex
  • Target synonyms
    HVPS41; hVps41p; HVSP41; S53; SCAR29; vacuolar assembly protein 41; vacuolar protein sorting 41 homolog; vacuolar protein sorting-associated protein 41 homolog; VPS41, HOPS complex subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VPS41 using anti-VPS41 antibody (A04135-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS41 antigen affinity purified polyclonal antibody (Catalog # A04135-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS41 at approximately 99 kDa. The expected band size for VPS41 is at 99 kDa.
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