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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA486228(VPS53 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA486228(VPS53 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA486228(VPS53 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

VPS53 Antibody

CSB-PA486228
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetVPS53
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Overview

  • Supplier
    Cusabio
  • Product Name
    VPS53 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55275
  • Target name
    VPS53
  • Target description
    VPS53 subunit of GARP complex
  • Target synonyms
    HCCS1, PCH2E, hVps53L, pp13624, vacuolar protein sorting-associated protein 53 homolog, VPS53, GARP complex subunit, hepatocellular carcinoma suppressor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VIR6
  • Protein Name
    Vacuolar protein sorting-associated protein 53 homolog
  • Scientific Description
    This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VPS53 using anti-VPS53 antibody (A06836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS53 antigen affinity purified polyclonal antibody (Catalog # A06836-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS53 at approximately 100 kDa. The expected band size for VPS53 is at 94 kDa.
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