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ProductsBack/VRK2 Polyclonal Antibody
Lane 1: mouse embryo lysates; Lane 2: mouse liver lysates probed with VRK2 Polyclonal Antibody, Unconjugated (bs-12780R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: mouse embryo lysates; Lane 2: mouse liver lysates probed with VRK2 Polyclonal Antibody, Unconjugated (bs-12780R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: mouse embryo lysates; Lane 2: mouse liver lysates probed with VRK2 Polyclonal Antibody, Unconjugated (bs-12780R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

VRK2 Polyclonal Antibody

BS-12780R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetVRK2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    VRK2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7444
  • Target name
    VRK2
  • Target description
    VRK serine/threonine kinase 2
  • Target synonyms
    serine/threonine-protein kinase VRK2; vaccinia related kinase 2; vaccinia virus B1R-related kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86Y07
  • Protein Name
    Serine/threonine-protein kinase VRK2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of VRK2 using anti-VRK2 antibody (A04821-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRK2 antigen affinity purified polyclonal antibody (Catalog # A04821-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRK2 at approximately 50 kDa. The expected band size for VRK2 is at 58 kDa.
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