Bio-Connect
Host: Rabbit Target Name: WDR46 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml
Host: Rabbit Target Name: WDR46 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml
Host: Rabbit Target Name: WDR46 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml

WDR46 Rabbit Polyclonal Antibody

TA358303
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetWDR46
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    WDR46 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID9277
  • Target name
    WDR46
  • Target description
    WD repeat domain 46
  • Target synonyms
    BING4; C6orf11; FP221; UTP7; WD repeat-containing protein 46; WD repeat-containing protein BING4
  • Host
    Rabbit
  • Protein IDO15213
  • Protein Name
    WD repeat-containing protein 46
  • Scientific Description
    WDR46 Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
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