More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/WIF1 antibody [6D27]

WIF1 antibody [6D27]

GTX53057
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWIF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    WIF1 antibody [6D27]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:500. IHC-P: 1:20 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6D27
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID11197
  • Target name
    WIF1
  • Target description
    Wnt inhibitory factor 1
  • Target synonyms
    WIF-1, wnt inhibitory factor 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y5W5
  • Protein Name
    Wnt inhibitory factor 1
  • Scientific Description
    The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Immunohistochemistry analysis of human testis tissue using CSB-PA026113LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
WIF1 AntibodyCSB-PA026113LA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetWIF1
  • SizePrice
WB analysis of various sample lysates using GTX16429 WIF1 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
WIF1 antibodyGTX16429
ApplicationsWestern Blot
TargetWIF1
  • SizePrice
IHC-P analysis of rat hippocampus tissue using GTX60226 WIF1 antibody.
Product group Antibodies
GeneTex
WIF1 antibodyGTX60226
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetWIF1
  • SizePrice
ICC/IF analysis of HeLa cells using GTX82765 WIF1 antibody [1G5]. Green : WIF1 Red: Actin filaments
Product group Antibodies
References
GeneTex
WIF1 antibody [1G5]GTX82765
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetWIF1
  • SizePrice
Product group Antibodies
MedChem Express
WIF1 Antibody (YA2664, Rabbit)HY-P82919
TargetWIF1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-WIF1 Antibody144-05386
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetWIF1
  • SizePrice
Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti WIF1 Polyclonal Antibody, Unconjugated (bs-3917R) followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss
WIF1 Polyclonal AntibodyBS-3917R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetWIF1
  • SizePrice
Product group Antibodies
Biomatik
WNT Inhibitory Factor 1 (WIF1) Polyclonal AntibodyCAU31894
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetWIF1
  • SizePrice
Product group Antibodies
Assay Genie
WIF1 AntibodyCAB12969
TargetWIF1
  • SizePrice
Figure 1. Western blot analysis of WIF1 using anti-WIF1 antibody (M03247-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WIF1 antigen affinity purified monoclonal antibody (Catalog # M03247-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WIF1 at approximately 42 kDa. The expected band size for WIF1 is at 42 kDa.
Product group Antibodies
Boster Bio
Anti-WIF1 Rabbit Monoclonal AntibodyM03247-1
ApplicationsImmunoPrecipitation, Western Blot
TargetWIF1
  • SizePrice