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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: WISP1 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 31, 14, 17, 22 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: WISP1 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 31, 14, 17, 22 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: WISP1 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 31, 14, 17, 22 kDa Observed band size: 41 kDa

WISP1 Antibody

CSB-PA026119LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCCN4
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Overview

  • Supplier
    Cusabio
  • Product Name
    WISP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8840
  • Target name
    CCN4
  • Target description
    cellular communication network factor 4
  • Target synonyms
    WISP1, WISP1-OT1, WISP1-UT1, WISP1c, WISP1i, WISP1tc, CCN family member 4, WISP1 3'UTR-associated RNA 1, WISP1 overlapping transcript 1 (non-protein coding), WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95388
  • Protein Name
    WNT1-inducible-signaling pathway protein 1
  • Scientific Description
    Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WISP-1/CCN4 using anti-WISP-1/CCN4 antibody (A03052-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WISP-1/CCN4 antigen affinity purified polyclonal antibody (Catalog # A03052-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WISP-1/CCN4 at approximately 48 kDa. The expected band size for WISP-1/CCN4 is at 55 kDa.
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