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Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody
Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody
Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody

WNT1 Antibody

CSB-PA004494
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetWNT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    WNT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7471
  • Target name
    WNT1
  • Target description
    Wnt family member 1
  • Target synonyms
    BMND16, INT1, OI15, proto-oncogene Wnt-1, proto-oncogene Int-1 homolog, wingless-type MMTV integration site family member 1, wingless-type MMTV integration site family, member 1 (oncogene INT1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04628
  • Protein Name
    Proto-oncogene Wnt-1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNT1 using anti-WNT1 antibody (PB9460). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT1 antigen affinity purified polyclonal antibody (Catalog # PB9460) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT1 at approximately 41 kDa. The expected band size for WNT1 is at 41 kDa.
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