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WNT2 antibody

70R-21324
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    WNT2 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT2 using anti-WNT2 antibody (PB9461). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: COLO320 Whole Cell Lysate at 40ug, Lane 2: MCF-7 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT2 antigen affinity purified polyclonal antibody (Catalog # PB9461) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT2 at approximately 40 kDa. The expected band size for WNT2 is at 40 kDa.
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Western blot All lanes: Protein Wnt-2 antibody at 2ug/ml Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
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Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4°C, DAB staining.
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