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WNT2 Monoclonal Antibody

BSM-60493M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetWNT2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    WNT2 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B3B10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7472
  • Target name
    WNT2
  • Target description
    Wnt family member 2
  • Target synonyms
    epididymis secretory sperm binding protein; INT1L1; int-1-like protein 1; Int-1-related protein; IRP; protein Wnt-2; secreted growth factor; wingless-type MMTV integration site family member 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09544
  • Protein Name
    Protein Wnt-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT2 using anti-WNT2 antibody (PB9461). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: COLO320 Whole Cell Lysate at 40ug, Lane 2: MCF-7 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT2 antigen affinity purified polyclonal antibody (Catalog # PB9461) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT2 at approximately 40 kDa. The expected band size for WNT2 is at 40 kDa.
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