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The data was published in the journal Sci Rep in 2019. PMID: 30914750
The data was published in the journal Sci Rep in 2019. PMID: 30914750
The data was published in the journal Sci Rep in 2019. PMID: 30914750

Wnt5b antibody, C-term

GTX47174
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetWNT5B
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Overview

  • Supplier
    GeneTex
  • Product Name
    Wnt5b antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID81029
  • Target name
    WNT5B
  • Target description
    Wnt family member 5B
  • Target synonyms
    protein Wnt-5b; wingless-type MMTV integration site family, member 5B; WNT-5B protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1J7
  • Protein Name
    Protein Wnt-5b
  • Scientific Description
    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Utilization of NGS technologies to investigate transcriptomic and epigenomic mechanisms in trastuzumab resistance. Nava M et al., 2019 Mar 26, Sci Rep
    Read more

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Figure 1. Western blot analysis of Wnt5b using anti-Wnt5b antibody (A05836-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt5b antigen affinity purified polyclonal antibody (Catalog # A05836-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt5b at approximately 45KD. The expected band size for Wnt5b is at 40KD.
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Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54708R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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