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Wnt5b Antibody (YA653)

HY-P80934
MedChem Express
TargetWNT5B
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Wnt5b Antibody (YA653)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID81029
  • Target name
    WNT5B
  • Target description
    Wnt family member 5B
  • Target synonyms
    protein Wnt-5b; wingless-type MMTV integration site family, member 5B; WNT-5B protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Wnt5b Antibody (YA653) is a non-conjugated and Mouse origined monoclonal antibody about 40 kDa, targeting to Wnt5b (3D1). It can be used for WB assays with tag free, in the background of Transfected.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Wnt5b using anti-Wnt5b antibody (A05836-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt5b antigen affinity purified polyclonal antibody (Catalog # A05836-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt5b at approximately 45KD. The expected band size for Wnt5b is at 40KD.
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The data was published in the journal Sci Rep in 2019. PMID: 30914750
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Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54708R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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