Titration of WRN.
WRN Helicase Activity Assay Kit
78852
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameWRN Helicase Activity Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN inhibitor NCGC00063279 is also included as a control. The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence.
- Storage InstructionSee manual
- UNSPSC41116133