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Western Blot analysis of COS7 cells using XRN2 Polyclonal Antibody
Western Blot analysis of COS7 cells using XRN2 Polyclonal Antibody
Western Blot analysis of COS7 cells using XRN2 Polyclonal Antibody

XRN2 Antibody

CSB-PA267042
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetXRN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    XRN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22803
  • Target name
    XRN2
  • Target description
    5'-3' exoribonuclease 2
  • Target synonyms
    5'-3' exoribonuclease 2, DHM1-like protein, DHP protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0D6
  • Protein Name
    5'-3' exoribonuclease 2
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XRN2 using anti-XRN2 antibody (A02797-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRN2 antigen affinity purified polyclonal antibody (Catalog # A02797-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRN2 at approximately 109KD. The expected band size for XRN2 is at 109KD.
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