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ProductsBack/Phospho-YB1 (Ser102) Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with YB1(Ser102) Polyclonal Antibody(bs-3477R)at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with YB1(Ser102) Polyclonal Antibody(bs-3477R)at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with YB1(Ser102) Polyclonal Antibody(bs-3477R)at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Phospho-YB1 (Ser102) Polyclonal Antibody

BS-3477R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetYBX1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    YB1(Ser102) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4904
  • Target name
    YBX1
  • Target description
    Y-box binding protein 1
  • Target synonyms
    BP-8; CBF-A; CCAAT-binding transcription factor I subunit A; CSDA2; CSDB; DBPB; DNA-binding protein B; EFI-A; enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR-NF1; NSEP1; NSEP-1; nuclease-sensitive element-binding protein 1; YB1; YB-1; Y-box transcription factor; Y-box-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP67809
  • Protein Name
    Nuclease-sensitive element-binding protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 5. IHC analysis of YB using anti-YB antibody (PB9465). YB was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-YB Antibody (PB9465) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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