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Western Blot analysis of COLO205 cells using MSY2 Polyclonal Antibody
Western Blot analysis of COLO205 cells using MSY2 Polyclonal Antibody
Western Blot analysis of COLO205 cells using MSY2 Polyclonal Antibody

YBX2 Antibody

CSB-PA003328
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetYBX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    YBX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51087
  • Target name
    YBX2
  • Target description
    Y-box binding protein 2
  • Target synonyms
    CONTRIN, CSDA3, DBPC, MSY2, Y-box-binding protein 2, DNA-binding protein C, alternative protein YBX2, epididymis secretory sperm binding protein, germ cell specific Y-box binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2T7
  • Protein Name
    Y-box-binding protein 2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MSY2/YBOX2/YBX2 using anti-MSY2/YBOX2/YBX2 antibody (A08610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSY2/YBOX2/YBX2 antigen affinity purified polyclonal antibody (Catalog # A08610-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSY2/YBOX2/YBX2 at approximately 55 kDa. The expected band size for MSY2/YBOX2/YBX2 is at 39 kDa.
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