More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/YES1 (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2B8]
Detection of endogenous yes Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab yes-2B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA231; lane 7: T47D
Detection of endogenous yes Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab yes-2B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA231; lane 7: T47D
Detection of endogenous yes Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab yes-2B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA231; lane 7: T47D

YES1 (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2B8]

AM00159PU-N
OriGene
ApplicationsWestern Blot, ELISA
DownloadMSDS
Product group Antibodies
TargetYES1
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    YES1 (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2B8]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2B8
  • Gene ID7525
  • Target name
    YES1
  • Target description
    YES proto-oncogene 1, Src family tyrosine kinase
  • Target synonyms
    cellular yes-1 protein; c-yes; HsT441; P61-YES; proto-oncogene c-Yes; proto-oncogene tyrosine-protein kinase YES; tyrosine-protein kinase Yes; v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; Yamaguchi sarcoma oncogene; Yes; YES1 proto-oncogene, Src family tyrosine kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07947
  • Protein Name
    Tyrosine-protein kinase Yes
  • Scientific Description
    YES1 (N-term) (incl. pos. control) mouse monoclonal antibody, clone 2B8, Purified
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-YES1 Antibody144-61627
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetYES1
  • SizePrice
Anti-Yes1 Antibody
Product group Antibodies
Antibodies.com
Anti-Yes1 AntibodyA12663
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
Biomatik
YES1 Polyclonal AntibodyCAC14604
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetYES1
  • SizePrice
Product group Antibodies
Assay Genie
YES1 AntibodyCAB0628
TargetYES1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
YES1 / c-Yes AntibodyLS-C500040
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetYES1
  • SizePrice
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate All lanes: YES1 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61 kDa Observed band size: 61, 70 kDa
Product group Antibodies
Cusabio
YES1 AntibodyCSB-PA026253LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetYES1
  • SizePrice
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.
Product group Antibodies
Atlas Antibodies
Anti-YES1 AntibodyHPA026480
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetYES1
  • SizePrice
Figure 1. Western blot analysis of YES1 using anti-YES1 antibody (PB9466). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YES1 antigen affinity purified polyclonal antibody (Catalog # PB9466) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YES1 at approximately 61 kDa. The expected band size for YES1 is at 61 kDa.
Product group Antibodies
Boster Bio
Anti-Yes1 Antibody Picoband(r)PB9466-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetYES1
  • SizePrice
Product group Antibodies
References
GeneTex
c-Yes antibodyGTX100616
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetYES1
  • SizePrice
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti YES1 Polyclonal Antibody, Unconjugated (bs-4166R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss Antibodies
YES1 Polyclonal AntibodyBS-4166R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetYES1
  • SizePrice