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Ythdf2 Polyclonal Antibody

CAC09978
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetYTHDF2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Ythdf2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51441
  • Target name
    YTHDF2
  • Target description
    YTH N6-methyladenosine RNA binding protein F2
  • Target synonyms
    CAHL, DF2, HGRG8, NY-REN-2, YTH domain-containing family protein 2, 9430020E02Rik, CLL-associated antigen KW-14, YTH N(6)-methyladenosine RNA binding protein 2, YTH N6-methyladenosine RNA binding protein 2, YTH domain family, member 2, high-glucose-regulated protein 8, renal carcinoma antigen NY-REN-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5A9
  • Protein Name
    YTH domain-containing family protein 2
  • Scientific Description
    The Ythdf2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of YTHDF2 using anti-YTHDF2 antibody (A02621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human Hepg2 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDF2 antigen affinity purified polyclonal antibody (Catalog # A02621-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDF2 at approximately 65KD. The expected band size for YTHDF2 is at 65KD.
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