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ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).

Zap70 (4C2) Monoclonal Antibody

BSM-51298M
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetZap70
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Overview

  • Supplier
    Bioss
  • Product Name
    Zap70 (4C2) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C2
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID22637
  • Target name
    Zap70
  • Target description
    zeta-chain (TCR) associated protein kinase
  • Target synonyms
    Srk, ZAP-70, mrtle, mur, tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, syk-related tyrosine kinase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP43404
  • Protein Name
    Tyrosine-protein kinase ZAP-70
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Zap70 with M00754-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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