WB (peptide competition) analysis of Jurkat cells treated with H2O2 (Lane 2-6) using GTX12869 ZAP70 (phospho Tyr315/Tyr319) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4) the phosphopeptide immunogen (Lane 5) or, the phosphopeptide corresponding to ZAP-70 (pY315) (Lane 6) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 7) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of Jurkat cells treated with H2O2 (Lane 2-6) using GTX12869 ZAP70 (phospho Tyr315/Tyr319) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4) the phosphopeptide immunogen (Lane 5) or, the phosphopeptide corresponding to ZAP-70 (pY315) (Lane 6) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 7) eliminates the signal further verifying that the antibody is phospho-specific.
ZAP70 (phospho Tyr315/Tyr319) antibody
GTX12869
Overview
- SupplierGeneTex
- Product NameZAP70 (phospho Tyr315/Tyr319) antibody - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID7535
- Target nameZAP70
- Target descriptionzeta chain of T cell receptor associated protein kinase 70
- Target synonyms70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
- HostRabbit
- IsotypeIgG
- Protein IDP43403
- Protein NameTyrosine-protein kinase ZAP-70
- Scientific DescriptionThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203