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The data was published in the journal EMBO Rep in 2017.
The data was published in the journal EMBO Rep in 2017.
The data was published in the journal EMBO Rep in 2017.

ZBTB48 antibody

GTX118671
GeneTex
ApplicationsImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetZBTB48
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZBTB48 antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3104
  • Target name
    ZBTB48
  • Target description
    zinc finger and BTB domain containing 48
  • Target synonyms
    HKR3, TZAP, ZNF855, pp9964, zinc finger and BTB domain-containing protein 48, GLI-Kruppel family member HKR3, krueppel-related zinc finger protein 3, telomere zinc finger-associated protein, telomere-binding protein and transcriptional activator ZBTB48, telomeric zinc finger-associated protein, zinc finger protein 855
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10074
  • Protein Name
    Zinc finger and BTB domain-containing protein 48
  • Scientific Description
    Binds to and regulates the J and/or S elements in MHC II promoter.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ZBTB48 using anti-ZBTB48 antibody (A13220-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB48 antigen affinity purified polyclonal antibody (Catalog # A13220-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB48 at approximately 77 kDa. The expected band size for ZBTB48 is at 77 kDa.
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