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ProductsBack/ZEB1/NIL2A Polyclonal Antibody
H9C2 cells probed with Rabbit Anti-ZEB Polyclonal Antibody (bs-4187R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 for 30 minutes (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange)
H9C2 cells probed with Rabbit Anti-ZEB Polyclonal Antibody (bs-4187R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 for 30 minutes (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange)
H9C2 cells probed with Rabbit Anti-ZEB Polyclonal Antibody (bs-4187R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 for 30 minutes (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange)

ZEB1/NIL2A Polyclonal Antibody

BS-4187R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetZEB1
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Overview

  • Supplier
    Bioss
  • Product Name
    ZEB Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6935
  • Target name
    ZEB1
  • Target description
    zinc finger E-box binding homeobox 1
  • Target synonyms
    AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A, zinc finger E-box-binding homeobox 1, delta-crystallin enhancer binding factor 1, negative regulator of IL2, posterior polymorphous corneal dystrophy 3, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60315
  • Protein Name
    Zinc finger E-box-binding homeobox 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of ZEB1 using anti-ZEB1 antibody (A00548-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HeLa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZEB1 antigen affinity purified polyclonal antibody (Catalog # A00548-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZEB1 at approximately 200 kDa. The expected band size for ZEB1 is at 124 kDa.
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