Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232). Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112232/GTX112232_40107_20151204_IP_w_23060500_918.webp "Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232). Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112232/GTX112232_40107_20151216_IFA_w_23060500_246.webp "ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ZHX2 antibody [C1C3]
GTX112232
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse
TargetZHX2
Overview
- SupplierGeneTex
- Product NameZHX2 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Gene ID22882
- Target nameZHX2
- Target descriptionzinc fingers and homeoboxes 2
- Target synonymsAFR1, RAF, zinc fingers and homeoboxes protein 2, AFP regulator 1, alpha-fetoprotein regulator 1, regulator of AFP, transcription factor ZHX2, zinc finger and homeodomain protein 2
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y6X8
- Protein NameZinc fingers and homeoboxes protein 2
- Scientific DescriptionThe members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li D, Wang W, Liu B, et al. Characterization of circSEC11A as a novel regulator of Iodine-125 radioactive seed-induced anticancer effects in hepatocellular carcinoma via targeting ZHX2/GADD34 axis. Cell Death Discov. 2023,9(1):294. doi: 10.1038/s41420-023-01593-wRead this paper
- He Y, Zhang Q, Chen Y, et al. ZHX2 deficiency enriches hybrid MET cells through regulating E-cadherin expression. Cell Death Dis. 2023,14(7):444. doi: 10.1038/s41419-023-05974-yRead this paper
- Xie H, Zhou J, Liu X, et al. USP13 promotes deubiquitination of ZHX2 and tumorigenesis in kidney cancer. Proc Natl Acad Sci U S A. 2022,119(36):e2119854119. doi: 10.1073/pnas.2119854119Read this paper
- Mondal S, Ramanathan M, Miao W, et al. PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Nat Methods. 2022,19(8):959-968. doi: 10.1038/s41592-022-01552-wRead this paper
- Chen Y, Zhu L, Xue S, et al. Novel VHL substrate targets SFMBT1 and ZHX2 may be important prognostic predictors in patients with ccRCC. Oncol Lett. 2021,21(5):379. doi: 10.3892/ol.2021.12640Read this paper
- Jiang J, Sun Y, Xu J, et al. ZHX2 mediates proteasome inhibitor resistance via regulating nuclear translocation of NF-κB in multiple myeloma. Cancer Med. 2020,9(19):7244-7252. doi: 10.1002/cam4.3347Read this paper
- Lee SB, Ko A, Oh YT, et al. Proline Hydroxylation Primes Protein Kinases for Autophosphorylation and Activation. Mol Cell. 2020,79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021Read this paper
- Zhu L, Ding R, Yan H, et al. ZHX2 drives cell growth and migration via activating MEK/ERK signal and induces Sunitinib resistance by regulating the autophagy in clear cell Renal Cell Carcinoma. Cell Death Dis. 2020,11(5):337. doi: 10.1038/s41419-020-2541-xRead this paper
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
Datasheet
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