Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232). Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112232/GTX112232_40107_20151204_IP_w_23060500_918.webp "Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232). Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112232/GTX112232_40107_20151216_IFA_w_23060500_246.webp "ZHX2 antibody [C1C3] detects ZHX2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 protein stained by ZHX2 antibody [C1C3] (GTX112232) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ZHX2 antibody [C1C3]
GTX112232
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse
TargetZHX2
Overview
- SupplierGeneTex
- Product NameZHX2 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Gene ID22882
- Target nameZHX2
- Target descriptionzinc fingers and homeoboxes 2
- Target synonymsAFR1, RAF, zinc fingers and homeoboxes protein 2, AFP regulator 1, alpha-fetoprotein regulator 1, regulator of AFP, transcription factor ZHX2, zinc finger and homeodomain protein 2
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y6X8
- Protein NameZinc fingers and homeoboxes protein 2
- Scientific DescriptionThe members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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