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ZNF198 antibody

GTX105550
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetZMYM2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZNF198 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:50-1:500. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.57 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7750
  • Target name
    ZMYM2
  • Target description
    zinc finger MYM-type containing 2
  • Target synonyms
    FIM, MYM, NECRC, RAMP, SCLL, ZNF198, zinc finger MYM-type protein 2, fused in myeloproliferative disorders protein, rearranged in an atypical myeloproliferative disorder, zinc finger protein 198, zinc finger, MYM-type 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBW7
  • Protein Name
    Zinc finger MYM-type protein 2
  • Scientific Description
    The protein encoded by this gene is a zinc finger protein that may act as a transcription factor. The encoded protein may be part of a BHC histone deacetylase complex. Translocation of this gene with the fibroblast growth factor receptor-1 gene (FGFR1) results in a fusion gene, which may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yamanaka S, Furihata H, Yanagihara Y, et al. Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation. Nat Commun. 2023,14(1):4683. doi: 10.1038/s41467-023-40385-9
    Read this paper
  • Yamanaka S, Horiuchi Y, Matsuoka S, et al. A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues. Nat Commun. 2022,13(1):183. doi: 10.1038/s41467-021-27818-z
    Read this paper

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Figure 1. Western blot analysis of RAMP/ZMYM2 using anti-RAMP/ZMYM2 antibody (A04648-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAMP/ZMYM2 antigen affinity purified polyclonal antibody (Catalog # A04648-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAMP/ZMYM2 at approximately 150 kDa. The expected band size for RAMP/ZMYM2 is at 155 kDa.
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