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Western Blot analysis of LOVO cells using ZP1 Polyclonal Antibody
Western Blot analysis of LOVO cells using ZP1 Polyclonal Antibody
Western Blot analysis of LOVO cells using ZP1 Polyclonal Antibody

ZP1 Antibody

CSB-PA008280
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ZP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22917
  • Target name
    ZP1
  • Target description
    zona pellucida glycoprotein 1
  • Target synonyms
    HEL163, OOMD, OOMD1, OZEMA1, ZPB1, zona pellucida sperm-binding protein 1, epididymis luminal protein 163, zona pellucida glycoprotein 1 (sperm receptor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60852
  • Protein Name
    Zona pellucida sperm-binding protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ZP1 using anti-ZP1 antibody (PB9970). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP1 antigen affinity purified polyclonal antibody (Catalog # PB9970) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP1 at approximately 70 kDa, 100 kDa. The expected band size for ZP1 is at 70 kDa.
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