More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/14-3-3 eta antibody [N1C3]
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 eta antibody [N1C3] (GTX113577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 eta antibody [N1C3] (GTX113577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 eta antibody [N1C3] (GTX113577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

14-3-3 eta antibody [N1C3]

GTX113577
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetYWHAH
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    14-3-3 eta antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7533
  • Target name
    YWHAH
  • Target description
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta
  • Target synonyms
    YWHA1, 14-3-3 protein eta, 14-3-3 eta, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04917
  • Protein Name
    14-3-3 protein eta
  • Scientific Description
    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5 UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western blot All lanes: YWHAH antibody at 2microg/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Product group Antibodies
Cusabio
YWHAH AntibodyCSB-PA02174A0RB
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetYWHAH
  • SizePrice
Product group Antibodies
Assay Genie
YWHAH AntibodyCAB9079
ReactivityHuman
TargetYWHAH
  • SizePrice
Product group Antibodies
RayBiotech
Anti-YWHAH Antibody144-61464
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetYWHAH
  • SizePrice
Anti-14-3-3 eta Antibody
Product group Antibodies
Antibodies.com
Anti-14-3-3 eta AntibodyA95166
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
14-3-3 eta Antibody (YA838, Mouse)HY-P80525
TargetYWHAH
  • SizePrice
Figure 1. Western blot analysis of YWHAH using anti-YWHAH antibody (A04219-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAH antigen affinity purified polyclonal antibody (Catalog # A04219-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YWHAH at approximately 28 kDa. The expected band size for YWHAH is at 28 kDa.
Product group Antibodies
Boster Bio
Anti-YWHAH Antibody Picoband(r)A04219-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAH
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
YWHAH / 14-3-3 Eta AntibodyLS-C410613
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetYWHAH
  • SizePrice
Product group Antibodies
Biomatik
Ywhah Polyclonal AntibodyCAC07214
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetYWHAH
  • SizePrice
Product group Antibodies
Bioss
14-3-3 eta Monoclonal AntibodyBSM-33888M
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetYWHAH
  • SizePrice
Product group Antibodies
Biosynth
YWHAH antibody70R-10462
  • SizePrice