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Anti-YWHAH Antibody

144-61464
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetYWHAH
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-YWHAH Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7533
  • Target name
    YWHAH
  • Target description
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta
  • Target synonyms
    YWHA1, 14-3-3 protein eta, 14-3-3 eta, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    YWHAH Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 eta antibody [N1C3] (GTX113577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of YWHAH using anti-YWHAH antibody (A04219-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAH antigen affinity purified polyclonal antibody (Catalog # A04219-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YWHAH at approximately 28 kDa. The expected band size for YWHAH is at 28 kDa.
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