More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/14-3-3 sigma Recombinant Antibody

14-3-3 sigma Recombinant Antibody

BSM-60449M
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSFN
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    14-3-3 sigma Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2810
  • Target name
    SFN
  • Target description
    stratifin
  • Target synonyms
    YWHAS, 14-3-3 protein sigma, epithelial cell marker protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31947
  • Protein Name
    14-3-3 protein sigma
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
ADAM19 AntibodyCAB10260
ReactivityHuman
TargetSFN
  • SizePrice
Product group Antibodies
Cusabio
SFN AntibodyCSB-PA008892
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetSFN
  • SizePrice
Anti-14-3-3 sigma/SFN Antibody (Biotin)
Product group Antibodies
Antibodies.com
Anti-14-3-3 sigma/SFN Antibody (Biotin)A84165
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat skin tissue lysates, Lane 6: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma at approximately 28 kDa. The expected band size for 14-3-3 sigma is at 28 kDa.
Product group Antibodies
Boster Bio
Anti-14-3-3 sigma/SFN Antibody Picoband(r)A01127-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSFN
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SFN / Stratifin / 14-3-3 Sigma AntibodyLS-C830366
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSFN
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-14-3-3 sigma / Stratifin, BiotinylatedEB07061-B
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Porcine, Rat
TargetSFN
  • SizePrice
Immunohistochemistry analysis in human skin and skeletal muscle tissues using Anti-SFN antibody. Corresponding SFN RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-SFN AntibodyHPA011105
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSFN
  • SizePrice
Product group Antibodies
Biomatik
SFN Polyclonal AntibodyCAC15119
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetSFN
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100289) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Product group Antibodies
GeneTex
14-3-3 sigma antibodyGTX100289
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetSFN
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SFN Antibody144-01026
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSFN
  • SizePrice