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ProductsBack/4E-BP1 antibody [N1C3-2]
Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

4E-BP1 antibody [N1C3-2]

GTX116315
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF4EBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    4E-BP1 antibody [N1C3-2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.09 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1978
  • Target name
    EIF4EBP1
  • Target description
    eukaryotic translation initiation factor 4E binding protein 1
  • Target synonyms
    4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, phosphorylated heat- and acid-stable protein regulated by insulin 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5 end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cardile A, Zanrè V, Campagnari R, et al. Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism. Int J Mol Sci. 2023,24(2). doi: 10.3390/ijms24021263
    Read this paper

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Figure 1. Western blot analysis of EIF4EBP1 using anti-EIF4EBP1 antibody (A00968-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4EBP1 antigen affinity purified polyclonal antibody (Catalog # A00968-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4EBP1 at approximately 17 kDa. The expected band size for EIF4EBP1 is at 17 kDa.
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