5-Ethynyl-cytidine (5-EC) [65223-78-1]

Jena Bioscience

5-Ethynyl-cytidine (5-EC) [65223-78-1]

5

65223-78-1

Research Use Only

≥99%

C11H13N3O5

267.24 g/mol

Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

n1(cc(c(nc1=O)N)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O

-20°C

12352200