5-Ethynyl-cytidine (5-EC)

Biorbyt

5-Ethynyl-cytidine (5-EC) [65223-78-1]

16

RNA synthesis monitoring. Spectroscopic Propertie: lambdamax 294 nm, epsilon 8.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

65223-78-1

Research Use Only

≥99%

Theoretical MW: 267.24 g/mol; Detected MW: 267.09 g/mol

Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.

-20°C

12352200