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ProductsBack/5-HT1A receptor antibody [N3C1], Internal

5-HT1A receptor antibody [N3C1], Internal

GTX104703
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse
TargetHTR1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    5-HT1A receptor antibody [N3C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.03 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3350
  • Target name
    HTR1A
  • Target description
    5-hydroxytryptamine receptor 1A
  • Target synonyms
    5-HT-1A, 5-HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G-21, PFMCD, 5-hydroxytryptamine receptor 1A, 5-HT1a receptor, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, guanine nucleotide-binding regulatory protein-coupled receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kim HJ, You MJ, Sung S, et al. Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression. Front Cell Neurosci. 2023,17:1162966. doi: 10.3389/fncel.2023.1162966
    Read this paper
  • Lim J, Bang Y, Kim KM, et al. Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors. Front Pharmacol. 2022,13:1062650. doi: 10.3389/fphar.2022.1062650
    Read this paper
  • Liang L, Zeng T, Zhao Y, et al. Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation. J Pineal Res. 2021,71(4):e12771. doi: 10.1111/jpi.12771
    Read this paper
  • Bhowmick S, Malat A, Caruso D, et al. Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress. eNeuro. 2021,8(4):pii: ENEURO.0242-21.2021. doi: 10.1523/ENEURO.0242-21.2021.
    Read this paper
  • Vegas-Suárez S, Aristieta A, Requejo C, et al. The effect of 5-HT(1A) receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats. Br J Pharmacol. 2021,178(12):2516-2532. doi: 10.1111/bph.15437
    Read this paper
  • Sugimoto K, Ichikawa-Tomikawa N, Nishiura K, et al. Serotonin/5-HT1A Signaling in the Neurovascular Unit Regulates Endothelial CLDN5 Expression. Int J Mol Sci. 2020,22(1). doi: 10.3390/ijms22010254
    Read this paper
  • Vegas-Suárez S, Pisanò CA, Requejo C, et al. 6-Hydroxydopamine lesion and levodopa treatment modify the effect of buspirone in the substantia nigra pars reticulata. Br J Pharmacol. 2020,177(17):3957-3974. doi: 10.1111/bph.15145
    Read this paper
  • Chen L, Yao Z, Qu S, et al. Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress. J Int Med Res. 2020,48(5):300060520918419. doi: 10.1177/0300060520918419
    Read this paper
  • Kim J, Lim J, Moon SH, et al. Toluene Inhalation Causes Early Anxiety and Delayed Depression with Regulation of Dopamine Turnover, 5-HT(1A) Receptor, and Adult Neurogenesis in Mice. Biomol Ther (Seoul). 2020,28(3):282-291. doi: 10.4062/biomolther.2020.055
    Read this paper
  • Rudyk C, Dwyer Z, Hayley S, et al. Leucine-rich repeat kinase-2 (LRRK2) modulates paraquat-induced inflammatory sickness and stress phenotype. J Neuroinflammation. 2019,16(1):120. doi: 10.1186/s12974-019-1483-7
    Read this paper

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Figure 1. Western blot analysis of HTR1A using anti-HTR1A antibody (PA1647). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR1A antigen affinity purified polyclonal antibody (Catalog # PA1647) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR1A at approximately 46 kDa. The expected band size for HTR1A is at 46 kDa.
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