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Western Blot analysis of Jurkat cells using SR-1A Polyclonal Antibody
Western Blot analysis of Jurkat cells using SR-1A Polyclonal Antibody
Western Blot analysis of Jurkat cells using SR-1A Polyclonal Antibody

HTR1A Antibody

CSB-PA004142
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHTR1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    HTR1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3350
  • Target name
    HTR1A
  • Target description
    5-hydroxytryptamine receptor 1A
  • Target synonyms
    5HT1a; 5-HT1A; 5-HT-1A; 5-HT1a receptor; 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled; 5-hydroxytryptamine receptor 1A; ADRB2RL1; ADRBRL1; G-21; guanine nucleotide-binding regulatory protein-coupled receptor; PFMCD
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HTR1A using anti-HTR1A antibody (PA1647). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR1A antigen affinity purified polyclonal antibody (Catalog # PA1647) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR1A at approximately 46 kDa. The expected band size for HTR1A is at 46 kDa.
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