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Immunohistochemical staining of human ovary shows weak cytoplasmic positivity in ovarian stroma cells.
Immunohistochemical staining of human ovary shows weak cytoplasmic positivity in ovarian stroma cells.
Immunohistochemical staining of human ovary shows weak cytoplasmic positivity in ovarian stroma cells.

Anti-HTR1A Antibody

HPA018073
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHTR1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HTR1A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3350
  • Target name
    HTR1A
  • Target description
    5-hydroxytryptamine receptor 1A
  • Target synonyms
    5-HT-1A, 5-HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G-21, PFMCD, 5-hydroxytryptamine receptor 1A, 5-HT1a receptor, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, guanine nucleotide-binding regulatory protein-coupled receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08908
  • Protein Name
    5-hydroxytryptamine receptor 1A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HTR1A using anti-HTR1A antibody (PA1647). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR1A antigen affinity purified polyclonal antibody (Catalog # PA1647) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR1A at approximately 46 kDa. The expected band size for HTR1A is at 46 kDa.
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