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IHC-P analysis of human vascular smooth muscle tissue using GTX13154 5-HT1B receptor antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human vascular smooth muscle tissue using GTX13154 5-HT1B receptor antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human vascular smooth muscle tissue using GTX13154 5-HT1B receptor antibody. Antigen retrieval : Heat-induced antigen retrieval

5-HT1B receptor antibody

GTX13154
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHTR1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    5-HT1B receptor antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 7 microg/ml. IHC-P: 7 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3351
  • Target name
    HTR1B
  • Target description
    5-hydroxytryptamine receptor 1B
  • Target synonyms
    5-HT1B; 5-HT-1B; 5-HT1DB; 5-HT-1D-beta; 5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled; 5-hydroxytryptamine receptor 1B; HTR1D2; HTR1DB; S12; serotonin 1D beta receptor; serotonin receptor 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs. [provided by RefSeq, Nov 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 5HT1B/HTR1B using anti-5HT1B/HTR1B antibody (A01826-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT1B/HTR1B antigen affinity purified polyclonal antibody (Catalog # A01826-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT1B/HTR1B at approximately 50 kDa. The expected band size for 5HT1B/HTR1B is at 50 kDa.
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