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Anti-HTR1B (Center) Antibody

102-21930
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetHTR1B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HTR1B (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB34552
  • Gene ID3351
  • Target name
    HTR1B
  • Target description
    5-hydroxytryptamine receptor 1B
  • Target synonyms
    5-HT1B; 5-HT-1B; 5-HT1DB; 5-HT-1D-beta; 5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled; 5-hydroxytryptamine receptor 1B; HTR1D2; HTR1DB; S12; serotonin 1D beta receptor; serotonin receptor 1B
  • Host
    Rabbit
  • Scientific Description
    Rabbit Anti-HTR1B (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 5HT1B/HTR1B using anti-5HT1B/HTR1B antibody (A01826-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT1B/HTR1B antigen affinity purified polyclonal antibody (Catalog # A01826-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT1B/HTR1B at approximately 50 kDa. The expected band size for 5HT1B/HTR1B is at 50 kDa.
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