_w_23053123_270.webp)
ICC/IF analysis of U2OS cells using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:100 Blue : DAPI for nuclear staining.
![IHC-P analysis of human appendix tissue section using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX02838/A2877_IHC_01_(1073265)_w_23053123_349.webp "IHC-P analysis of human appendix tissue section using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:100")
![IHC-P analysis of mouse spleen tissue section using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX02838/A2877_IHC_02_(1073266)_w_23053123_205.webp "IHC-P analysis of mouse spleen tissue section using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:100")
![WB analysis of Raji whole cell lysate using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02838/CutImage_A2877_WB_01_(1073922)_w_23053123_674.webp "WB analysis of Raji whole cell lysate using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:1000 Loading : 25microg")
![WB analysis of rat lung tissue lysates using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02838/CutImage_A2877_WB_02_(1073923)_w_23053123_113.webp "WB analysis of rat lung tissue lysates using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:1000 Loading : 25microg")
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5-Lipoxygenase antibody [GT1241] (GTX02838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02838/GTX02838_4000001926_20210122_WB_2_w_23053123_294.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5-Lipoxygenase antibody [GT1241] (GTX02838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with 5-Lipoxygenase antibody [GT1241] (GTX02838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02838/GTX02838_4000001926_20210122_WB_w_23053123_998.webp "Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with 5-Lipoxygenase antibody [GT1241] (GTX02838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ICC/IF analysis of U2OS cells using GTX02838 5-Lipoxygenase antibody [GT1241]. Dilution : 1:100 Blue : DAPI for nuclear staining.
5-Lipoxygenase antibody [GT1241]
GTX02838
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALOX5
Overview
- SupplierGeneTex
- Product Name5-Lipoxygenase antibody [GT1241]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1241
- Concentration1.45 mg/ml
- ConjugateUnconjugated
- Gene ID240
- Target nameALOX5
- Target descriptionarachidonate 5-lipoxygenase
- Target synonyms5-LO; 5-LOX; 5LPG; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; LOG5; LOX-5; polyunsaturated fatty acid 5-lipoxygenase
- HostRabbit
- IsotypeIgG
- Protein IDP09917
- Protein NameArachidonate 5-lipoxygenase
- Scientific DescriptionThis gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203