5-Vinyl-2-deoxyuridine (5-VdU) [55520-67-7]

Biorbyt

5-Vinyl-2-deoxyuridine (5-VdU) [55520-67-7]

16

DNA synthesis monitoring. Spectroscopic Propertie: lambdamax 292 nm, epsilon 9.2 L mmol-1 cm-1, (Methanol).

55520-67-7

Research Use Only

≥98%

Theoretical MW: 254.24 g/mol; Detected MW: 254.09 g/mol

5-VdU (5-Vinyl-2-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

-20°C

12352200