5-Vinyl-2-deoxyuridine (5-VdU) [55520-67-7]
ORB532301
CAS Number55520-67-7
Product group Chemicals
Estimated Purity≥98%
Molecular WeightTheoretical MW: 254.24 g/mol; Detected MW: 254.09 g/mol
Overview
- SupplierBiorbyt
- Product Name5-Vinyl-2-deoxyuridine (5-VdU) [55520-67-7]
- Delivery Days Customer16
- Application Supplier NoteDNA synthesis monitoring. Spectroscopic Propertie: lambdamax 292 nm, epsilon 9.2 L mmol-1 cm-1, (Methanol).
- CAS Number55520-67-7
- CertificationResearch Use Only
- Estimated Purity≥98%
- Molecular WeightTheoretical MW: 254.24 g/mol; Detected MW: 254.09 g/mol
- Scientific Description5-VdU (5-Vinyl-2-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.
- Storage Instruction-20°C
- UNSPSC12352200