5-Vinyl-uridine (5-VU), 5-Ethenyl-uridine [55520-64-4]

Jena Bioscience

5-Vinyl-uridine (5-VU), 5-Ethenyl-uridine [55520-64-4]

5

55520-64-4

Research Use Only

≥98%

C11H14N2O6

270.24 g/mol

5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2-deoxyuridine) for DNA synthesis monitoring[1]. The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

n1(cc(c(=O)[nH]c1=O)C=C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O

-20°C

12352200