Carboxy-DCFDA

Chemodex

5(6)-Carboxy-DCFDA [127770-45-0]

10

127770-45-0

Research Use Only

>85%

Warning

C25H14Cl2O9

529.28

Chemical. CAS: 127770-45-0. Formula: C25H14Cl2O9. MW: 529.28. Synthetic. Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH. - Non-activated, amine-reactive fluorescent probe. Mixture of 5/6-isomers of CDCFDA that can be used in biomolecules conjugation. 5(6)-CDCFDA is an amine-reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. 5(6)-CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. 5(6)-CDCFDA does not stain dead cells. 5(6)-CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: lambdaex=504nm, lambdaem=529nm.

CC(O)=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2

2°C to 8°C

41116134