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ProductsBack/A1BG (F-9): m-IgG Fc BP-HRP Bundle

A1BG (F-9): m-IgG Fc BP-HRP Bundle

SC-527461
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetA1BG
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    A1BG (F-9): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1
  • Target name
    A1BG
  • Target description
    alpha-1-B glycoprotein
  • Target synonyms
    A1B, ABG, GAB, HYST2477, alpha-1B-glycoprotein, HEL-S-163pA, epididymis secretory sperm binding protein Li 163pA
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha 1B-Glycoprotein/A1BG using anti-Alpha 1B-Glycoprotein/A1BG antibody (A07289-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat plasma lysates, Lane 4: mouse plasma lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG antigen affinity purified polyclonal antibody (Catalog # A07289-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1B-Glycoprotein/A1BG at approximately 50-75 kDa. The expected band size for Alpha 1B-Glycoprotein/A1BG is at 54 kDa.
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