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Western blot analysis of extracts of various cell lines using A1BG Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using A1BG Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using A1BG Polyclonal Antibody at dilution of 1:3000.

A1BG Polyclonal Antibody

E-AB-64079
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    A1BG Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha 1B-Glycoprotein/A1BG using anti-Alpha 1B-Glycoprotein/A1BG antibody (A07289-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat plasma lysates, Lane 4: mouse plasma lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG antigen affinity purified polyclonal antibody (Catalog # A07289-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1B-Glycoprotein/A1BG at approximately 50-75 kDa. The expected band size for Alpha 1B-Glycoprotein/A1BG is at 54 kDa.
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Lane 1: MBA-MB-231 cell lysates; Lane 2: Raji cell lysates probed with A1BG Polyclonal Antibody, Unconjugated (bs-6772R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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