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Western Blot analysis of Hela cells using AATF Polyclonal Antibody
Western Blot analysis of Hela cells using AATF Polyclonal Antibody
Western Blot analysis of Hela cells using AATF Polyclonal Antibody

AATF Antibody

CSB-PA001612
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAATF
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Overview

  • Supplier
    Cusabio
  • Product Name
    AATF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26574
  • Target name
    AATF
  • Target description
    apoptosis antagonizing transcription factor
  • Target synonyms
    BFR2; CHE1; CHE-1; DED; protein AATF; rb-binding protein Che-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NY61
  • Protein Name
    Protein AATF
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (Catalog # A03945-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
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