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Anti-AATF Antibody

CAB5896
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetAATF
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-AATF Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID26574
  • Target name
    AATF
  • Target description
    apoptosis antagonizing transcription factor
  • Target synonyms
    BFR2, CHE-1, CHE1, DED, protein AATF, rb-binding protein Che-1
  • Host
    Rabbit
  • Protein IDQ9NY61
  • Protein Name
    Protein AATF
  • Scientific Description
    AATF Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (Catalog # A03945-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
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