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ABCA4 (Rim Protein) Antibody

BSM-70010M
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAbca4
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Overview

  • Supplier
    Bioss
  • Product Name
    ABCA4 (Rim Protein) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F4
  • Conjugate
    Unconjugated
  • Gene ID11304
  • Target name
    Abca4
  • Target description
    ATP-binding cassette, sub-family A member 4
  • Target synonyms
    Abc10, Abcr, D430003I15Rik, RmP, retinal-specific phospholipid-transporting ATPase ABCA4, ATP-binding cassette 10, ATP-binding cassette, sub-family A (ABC1), member 4, RIM ABC transporter, Rim protein, retinal-specific ATP-binding cassette transporter
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO35600
  • Protein Name
    Retinal-specific ATP-binding cassette transporter
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
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