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WB analysis of 50ug mouse brain tissue lysate using GTX12302 ABCA4 antibody. Dilution : 0.5 microg/mL
WB analysis of 50ug mouse brain tissue lysate using GTX12302 ABCA4 antibody. Dilution : 0.5 microg/mL
WB analysis of 50ug mouse brain tissue lysate using GTX12302 ABCA4 antibody. Dilution : 0.5 microg/mL

ABCA4 antibody

GTX12302
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetAbca4
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Overview

  • Supplier
    GeneTex
  • Product Name
    ABCA4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID11304
  • Target name
    Abca4
  • Target description
    ATP-binding cassette, sub-family A (ABC1), member 4
  • Target synonyms
    Abc1; Abc10; Abcr; ATP-binding cassette 10; ATP-binding cassette sub-family A member 4; AW050280; D430003I15Rik; R; retinal-specific ATP-binding cassette transporter; retinal-specific phospholipid-transporting ATPase ABCA4; RIM ABC transporter; Rim protein; RmP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO35600
  • Protein Name
    Retinal-specific ATP-binding cassette transporter
  • Scientific Description
    The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein was the first of the ABC transporters to be observed in photoreceptors and may play a role in the photoresponse. Mutations in the human gene are found in patients diagnosed with Stargardt disease and are associated with retinitis pigmentosa-19 and macular degeneration age-related 2. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
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